Related Experiment Video
Updated: Nov 1, 2025

Facilitating Drug Discovery: An Automated High-content Inflammation Assay in Zebrafish
Published on: July 16, 2012
Anti-Inflammatory Effect of Deflazacort
Abstract:
We compared the effects of deflazacort (DFZ) a methyloxazoline derivative of prednisolone (PRED), PRED and dexamethasone (DEX) on the IgE-mediated release of preformed (histamine) and de novo synthesized (leukotriene C4 (LTC4)) proinflammatory mediators from human peripheral blood basophils. Eighteen-hour incubation of basophils with pharmacological concentrations of DFZ (10--8 to 3 × 10--6M)concentration dependently inhibited (9-50%) the IgE-dependent release of histamine and LTC4 from basophils. DFZ had a similar anti-inflammatory efficacy as DEX (10--9 to 10--6M)and similar efficacy and potency as PRED (10--8 to 3 × 10--6M). We also evaluated the effects of DFZ (10--8 to 10--7M)in combination with cyclosporin A (CsA) on the IgE-mediated histamine and LTC4 release from basophils. Eighteen-hour incubation of basophils with low concentrations of DFZ (10--8 to 10--7M) produced an additive inhibition of the release of histamine and LTC4 caused by short-term incubation with CsA (30 ng/ml).
Related Concept Videos
Drugs for Treatment of Crohn's Disease in IBD Using Glucocorticoids
Drugs for Treatment of Crohn's Disease in IBD Using Immunomodulatory Agents
Drugs for Treatment of Ulcerative Colitis in IBD
Drugs for Treatment of Crohn's Disease in IBD Using Biologic Agents: Anti-TNF
Inflammatory Bowel Disease IV: Pharmacological Management
Pharmacologic...
Antiasthma Drugs: Inhaled Corticosteroids and Glucocorticoids
ICS work through a multifaceted mechanism of action. They suppress the inflammatory response caused by the proliferation of TH cells. They also reduce the transcription of the IL-2 gene, which is involved in the...

