Related Experiment Videos
Regulation of human IgE synthesis
The Journal of Investigative Dermatology
|July 1, 1985
Summary
Individuals with allergies can produce Immunoglobulin E (IgE) antibodies, unlike healthy individuals. T cells play a crucial role in regulating IgE production, particularly in allergic responses.
Area of Science:
- Immunology
- Allergy Research
- Cellular Biology
Background:
- Allergic individuals can mount an Immunoglobulin E (IgE) antibody response to antigens, differentiating them from normal individuals.
- High serum IgE levels are frequently associated with T cell immunodeficiencies, suggesting T cell involvement in human IgE regulation.
- Peripheral blood lymphocytes (PBL) from allergic individuals spontaneously secrete IgE in vitro, while normal PBL do not.
Purpose of the Study:
- To investigate the role of T cells in regulating IgE synthesis in allergic individuals.
- To explore the mechanisms by which T cells influence IgE production by B cells.
- To characterize IgE binding factors and their impact on IgE synthesis.
Main Methods:
- Culture of normal and allergic peripheral blood lymphocytes (PBL) and B cells with T cell clones.
- Analysis of IgE secretion induced by T cell activation and bystander effects.
- Isolation and characterization of T cells with Fc receptors for IgE (FcR+ T cells) and their secreted IgE binding factors.
- Phenotypic analysis of FcR+ IgE-potentiating T cell lines (T3+T4+Ia+).
Main Results:
- Normal B cells require T cell activation for IgE synthesis, unlike other immunoglobulin isotypes.
- Allergic B cells exhibit bystander IgE secretion when cultured with T cell clones.
- FcR+ T cells from hyper IgE syndrome patients secrete IgE-potentiating factors that enhance IgE synthesis in preactivated B cells from allergic subjects.
- IgE binding factors from normal serum suppress IgE synthesis, while those from hyper IgE syndrome patients possess both potentiating and suppressor activities.
Conclusions:
- T cells are critical for activating IgE B cells in allergic individuals.
- IgE B cell antibody secretion is modulated by IgE binding factors, with distinct activities observed in allergic versus normal individuals and in hyper IgE syndrome.
- Dysregulation of T cell-derived IgE binding factors may contribute to allergic diseases and hyper IgE syndrome.