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Related Concept Videos

CRISPR01:59

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR and crRNAs02:53

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
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CRISPR/Cas9 Genome Editing01:28

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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Clinical Applications of Epidermal Stem Cells01:19

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Epidermal stem cells (EpiSCs) are mainly located at the basal layer of the epidermis. These cells repair minor injuries of the skin and replace dead skin cells. However, EpiSCs’ cannot heal severe wounds such as major burns or those from diabetes or hereditary disorders. In such cases, culturing the epidermal stem cells from the patient is possible and has yielded successful treatment options, such as laboratory-grown skin grafts. These grafts are synthesized using a patient’s own...
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Adult Stem Cells01:33

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Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously...
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Psychodynamic Perspectives on Personality01:27

Psychodynamic Perspectives on Personality

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The psychodynamic perspective in psychology asserts that most personality functions operate unconsciously, outside of awareness. This means that the motives and emotions driving behavior often remain hidden, automatically buried in the unconscious mind as a defense mechanism to shield us from psychological distress. According to this theory, the unconscious mind contains thoughts, memories, and emotions that are too disturbing to face directly.
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Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9
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CRISPR/Cas9 in Stem Cell Research: Current Application and Future Perspective.

Sathya Narayanan Patmanathan1, Nareshwaran Gnanasegaran2, Moon Nian Lim1

  • 1Haematology Unit, Cancer Research Centre, Institute for Medical Research, Pahang Road, 50588 Kuala Lumpur, Malaysia.

Current Stem Cell Research & Therapy
|June 14, 2018
PubMed
Summary

The CRISPR/Cas9 genome editing tool enhances stem cell research and therapy. This technology enables precise genetic modification for disease modeling, drug screening, and potential clinical applications.

Keywords:
CRISPR/Cas9disease modelingdrug screeninggenome editingstem cellstargeted therapytoxicology.

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Area of Science:

  • Biotechnology
  • Genomics
  • Stem Cell Biology

Background:

  • The CRISPR/Cas9 system is a highly effective genome editing technology.
  • It is extensively utilized across various life science research fields.
  • CRISPR/Cas9 combined with stem cells offers powerful applications in disease modeling and therapeutics.

Purpose of the Study:

  • To review the current applications of CRISPR/Cas9 technology in stem cell research.
  • To explore the future prospects of CRISPR/Cas9 in stem cell-based therapies.
  • To highlight the synergy between genome editing and stem cell pluripotency.

Main Methods:

  • Genetic modification of pluripotent and multipotent stem cells using CRISPR/Cas9.
  • Differentiation of genetically modified stem cells into specific cell types.
  • Functional analysis and evaluation for potential clinical transplantation.

Main Results:

  • CRISPR/Cas9 facilitates robust genome editing in stem cells.
  • The technology enables the generation of diverse cell types for research and therapy.
  • Advancements have expanded the scope of stem cell applications.

Conclusions:

  • CRISPR/Cas9 technology is a transformative tool in stem cell research.
  • Its application holds significant promise for developing novel therapeutic strategies.
  • Continued advancements will further broaden its impact on regenerative medicine.