Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Chromosome Replication02:31

Chromosome Replication

10.7K
Before a cell can divide, it must accurately replicate all of its chromosomes, including the DNA and its associated histone and non-histone proteins.  This process begins at numerous origins of replication during the S phase of the cell cycle in each of a cell’s chromosomes simultaneously. Certain nucleotides can act as origins of replication, but these sequences are not well defined - especially in complex, multi-cellular, eukaryotic species. The length of DNA that spans an origin...
10.7K
Psychosexual Stages of Personality: Latency01:16

Psychosexual Stages of Personality: Latency

1.3K
Following the phallic stage in Freud's theory of psychosexual development, children enter a phase called the latency period, which lasts from approximately six to twelve years of age. Unlike earlier stages, where sexual impulses played a central role, Freud believed these impulses are repressed during the latency period, becoming part of the unconscious. This stage is often described as a time of psychological calm after the turbulence of the phallic stage.
The latency period is not...
1.3K
DNA Replication02:40

DNA Replication

59.7K
DNA replication involves the separation of the two strands of the double helix, with each strand serving as a template from which the new complementary strand is copied.  After replication, each double-stranded DNA includes one parental or “old” strand and one “new” strand. This is known as semiconservative replication. The resulting DNA molecules have the same sequence and are divided equally into the two daughter cells.
Replication in Prokaryotes
DNA replication...
59.7K
Replication in Prokaryotes02:35

Replication in Prokaryotes

98.8K
Overview
98.8K
Replication in Eukaryotes02:31

Replication in Eukaryotes

205.6K
Overview
205.6K
Genomics02:02

Genomics

40.8K
Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
40.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

HBV Envelope Protein-Bearing Vesicles Show Preferential Uptake in Hepatocyte-Derived Cells.

International journal of molecular sciences·2026
Same author

Potential for Core Fucose-Targeted Therapy Against HBV Infection of Human Normal Hepatocytes.

Viruses·2025
Same author

Cell-intrinsic regulation of HBV RNAs by the nonsense-mediated mRNA decay pathway controls viral replication.

iScience·2025
Same author

Analysis of the Functional Role of TIMM29 in the Hepatitis B Virus Life Cycle.

Microbiology and immunology·2025
Same author

MicroRNA-3145 as a potential therapeutic target for hepatitis B virus: inhibition of viral replication via downregulation of HBS and HBX.

Frontiers in microbiology·2025
Same author

Generation of Replication-Competent Hepatitis B Virus Harboring Tagged Polymerase for Visualization and Quantification of the Infection.

Microbiology and immunology·2024
Same journal

Peptidomics in the Spotlight: Advanced Sample Treatment Techniques and Analytical Insights.

Advances in experimental medicine and biology·2026
Same journal

Methods for the Investigation of Protein-Ligands Interactions.

Advances in experimental medicine and biology·2026
Same journal

Sample Preparation Strategies for Microbial Cell Surface Proteomics: Integrating Shaving and Shotgun Approaches.

Advances in experimental medicine and biology·2026
Same journal

Proteomic Sample Preparation for the Petroleum Industry: A Biocorrosion Case Study.

Advances in experimental medicine and biology·2026
Same journal

Proteomic and Functional Comparison of Extracellular Vesicles from Wild-Type and Lyn-Deficient Stromal Cells.

Advances in experimental medicine and biology·2026
Same journal

Proteomic Analysis of Histone Sequence Variants and Post-translationally Modified Forms.

Advances in experimental medicine and biology·2026
See all related articles

Related Experiment Video

Updated: Feb 9, 2026

Humanized NOD/SCID/IL2rγnull (hu-NSG) Mouse Model for HIV Replication and Latency Studies
07:10

Humanized NOD/SCID/IL2rγnull (hu-NSG) Mouse Model for HIV Replication and Latency Studies

Published on: January 7, 2019

16.4K

KSHV Genome Replication and Maintenance in Latency.

Keiji Ueda1

  • 1Division of Virology, Department of Microbiology and Immunology, Osaka University Graduate School of Medicine, Osaka, Japan. kueda@virus.med.osaka-u.ac.jp.

Advances in Experimental Medicine and Biology
|June 14, 2018
PubMed
Summary
This summary is machine-generated.

Kaposi's sarcoma-associated herpesvirus (KSHV), or human herpesvirus-8, establishes latency in target cells, expressing limited genes. This review covers KSHV replication and gene expression during latency.

Keywords:
Kaposin (K12)Kaposi’s sarcoma (KS)Kaposi’s sarcoma-associated herpesvirus (KSHV) or human herpesvirus-8 (HHV-8)LANA (ORF73)LatencyMulticentric Castleman’s disease (MCD)Primary effusion lymphoma (PEL)ori-Pv-FLIP (K13)v-cyclin (v-CYC, ORF72)

More Related Videos

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement
08:06

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement

Published on: January 19, 2017

8.8K
Quantitative Fluorescence In Situ Hybridization FISH and Immunofluorescence IF of Specific Gene Products in KSHV-Infected Cells
06:21

Quantitative Fluorescence In Situ Hybridization FISH and Immunofluorescence IF of Specific Gene Products in KSHV-Infected Cells

Published on: August 27, 2019

13.2K

Related Experiment Videos

Last Updated: Feb 9, 2026

Humanized NOD/SCID/IL2rγnull (hu-NSG) Mouse Model for HIV Replication and Latency Studies
07:10

Humanized NOD/SCID/IL2rγnull (hu-NSG) Mouse Model for HIV Replication and Latency Studies

Published on: January 7, 2019

16.4K
Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement
08:06

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement

Published on: January 19, 2017

8.8K
Quantitative Fluorescence In Situ Hybridization FISH and Immunofluorescence IF of Specific Gene Products in KSHV-Infected Cells
06:21

Quantitative Fluorescence In Situ Hybridization FISH and Immunofluorescence IF of Specific Gene Products in KSHV-Infected Cells

Published on: August 27, 2019

13.2K

Area of Science:

  • Virology
  • Oncology
  • Molecular Biology

Background:

  • Kaposi's sarcoma-associated herpesvirus (KSHV), also known as human herpesvirus-8 (HHV-8), is a gamma herpesvirus.
  • KSHV is linked to cancers including Kaposi's sarcoma, primary effusion lymphoma (PEL), and multicentric Castleman's disease.
  • Gamma herpesviruses, unlike other subfamilies, establish latency as a default infection strategy.

Purpose of the Study:

  • To review KSHV replication during latency.
  • To discuss KSHV gene expression in the latent phase.
  • To explore the mechanisms of viral genome maintenance in infected cells.

Main Methods:

  • Review of scientific literature on KSHV biology.
  • Analysis of KSHV gene expression during latency.
  • Examination of viral replication and genome segregation mechanisms.

Main Results:

  • During latency, KSHV expresses a limited set of genes, including LANA, v-cyclin, v-FLIP, kaposin, and 25 microRNAs.
  • KSHV utilizes host cell machinery for replication, employing a viral replication origin (ori-P) and LANA.
  • Replicated viral genomes are equally segregated to daughter cells, maintaining consistent copy numbers per cell.

Conclusions:

  • KSHV latency involves controlled gene expression and efficient utilization of host cell machinery.
  • Understanding KSHV replication and gene expression in latency is crucial for developing targeted therapies.
  • The virus's strategy ensures genome stability and propagation within host cells.