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Whole Mount Immunohistochemistry in Zebrafish Embryos and Larvae
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Whole Mount Immunohistochemistry in Zebrafish Embryos and Larvae

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General Whole-Mount Immunohistochemistry of Zebrafish (Danio rerio) Embryos and Larvae Protocol

Dércia Santos1, Sandra M Monteiro1, Ana Luzio2

  • 1Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), Universidade de Trás-os-Montes e Alto Douro (UTAD), Vila Real, Portugal.

Methods in Molecular Biology (Clifton, N.J.)
|June 14, 2018
PubMed

Immunohistochemistry (IHC) is a powerful research tool to localize specific antigens in whole-mount or tissue sections of embryos with labeled antibodies based on antigen-antibody interactions. In whole-mount IHC, the distribution of an antigen can be mapped rapidly and reliably in the embryos. Immunofluorescence microscopy is one of the numerous IHC methods that may be used to assess both the localization and endogenous levels of proteins of interest. In this chapter, a general protocol for whole-mount immunofluorescent labeling of zebrafish embryos and larvae is described.

Keywords:
AntibodyConfocal microscopyDanio rerioImmunofluorescenceProtein

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