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Proteomic Sample Preparation from Formalin Fixed and Paraffin Embedded Tissue
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⿿Comparing the proteome of snap frozen, RNAlater preserved, and formalin-fixed paraffin-embedded human tissue samples

Tue Bjerg Bennike1,2, Kenneth Kastaniegaard2, Simona Padurariu3

  • 1Research Unit for Molecular Diagnostic and Clinical Research, Hospital of Southern Jutland, Aabenraa, Denmark.

Eupa Open Proteomics
|June 15, 2018
PubMed

Keywords:
CAN, acetonitrileDF, directly-frozenFA, formic acidFASP, filter-aided sample preparationFDR, false discovery rateFFPE, formalin-fixedFormalin-fixedHLA-A class I, histocompatibility antigen A-23 alpha chainHLA-DRB1 class II, histocompatibility antigen DRB1-4 beta chainHuman colon mucosaLFQ, label-free quantificationMass spectrometryPCA, principle component analysisPSM, peptide spectral matchPTM, post-translational modificationParaffin-embeddedPreservationProteomicsRNAlaterSDC, sodium deoxycholateSDS, sodium dodecyl sulfateTEAB, triethylammonium bicarbonateiFFPE, immediately formalin-fixeds, standard deviationsFFPE, stored for 30 min prior to formalin-fixed

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