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Updated: Feb 9, 2026

A Method for Characterizing Embryogenesis in Arabidopsis
Published on: August 4, 2017
Phenotypic characterization of an Arabidopsis T-DNA insertion line SALK_063500
Natasha J Sng1, Anna-Lisa Paul1,2, Robert J Ferl1,2,3
1Plant Molecular and Cellular Biology, University of Florida, Fifield Hall, 2550 Hull Road, Gainesville, FL 32611, USA.
Abstract:
In this article we report the identification of a homozygous lethal T-DNA (transfer DNA) line within the coding region of the At1G05290 gene in the genome of Arabidopsis thaliana (Arabidopsis) line, SALK_063500. The T-DNA insertion is found within exon one of the AT1G05290 gene, however a homozygous T-DNA allele is unattainable. In the heterozygous T-DNA allele the expression levels of AT1G05290 were compared to wild type Arabidopsis (Col-0, Columbia). Further analyses revealed an aberrant silique phenotype found in the heterozygous SALK_063500 plants that is attributed to the reduced rate of pollen tube germination. These data are original and have not been published elsewhere.
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