Related Experiment Video
Updated: Feb 9, 2026

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition
Published on: October 9, 2014
An SPR based immunoassay for the sensitive detection of the soluble epithelial marker E-cadherin
Daniele Vergara1, Monica Bianco2, Rosanna Pagano3
1Department of Biological and Environmental Sciences and Technologies, University of Salento, Lecce, Italy; Laboratory of Clinical Proteomic, "Giovanni Paolo II" Hospital, ASL-, Lecce, Italy.
Abstract:
Protein biomarkers are important diagnostic tools for cancer and several other diseases. To be validated in a clinical context, a biomarker should satisfy some requirements including the ability to provide reliable information on a pathological state by measuring its expression levels. In parallel, the development of an approach capable of detecting biomarkers with high sensitivity and specificity would be ideally suited for clinical applications. Here, we performed an immune-based label free assay using Surface Plasmon Resonance (SPR)-based detection of the soluble form of E-cadherin, a cell-cell contact protein that is involved in the maintaining of tissue integrity. With this approach, we obtained a specific and quantitative detection of E-cadherin from a few hundred microliters of serum of breast cancer patients by obtaining a 10-fold enhancement in the detection limit over a traditional colorimetric ELISA.
More Related Videos
Related Concept Videos
Structure of Cadherins
Factors Affecting Solubility
Solubility Equilibria
The...
Cadherins in Tissue Organization
Cell Sorting During Development
Cell sorting plays an...
Solubility of Ionic Compounds
Physical Properties Affecting Solubility
As for any solution, the solubility of a gas in a liquid is affected by the attractive intermolecular forces between solute and solvent species. Unlike solid and liquid solutes, however, there is no solute-solute intermolecular attraction to overcome when a gaseous solute dissolves in a liquid solvent since the atoms or molecules comprising a gas are far separated and experience negligible interactions. Consequently, solute-solvent interactions are the sole...

