Related Experiment Video

Updated: Feb 8, 2026

Preparation of a Blood Culture Pellet for Rapid Bacterial Identification and Antibiotic Susceptibility Testing
11:25

Preparation of a Blood Culture Pellet for Rapid Bacterial Identification and Antibiotic Susceptibility Testing

Published on: October 15, 2014

36.6K

Application of BioFire FilmArray Blood Culture Identification panel for rapid identification of the causative agents

M R Pulido1, P Moreno-Martínez1, V González-Galán1

  • 1Clinical Unit of Infectious Diseases, Clinical Microbiology and Preventive Medicine, Seville, Spain.

Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases
|June 16, 2018
PubMed
Abstract

Insights

The BioFire FilmArray Blood Culture Identification (BCID) panel can quickly identify pathogens causing ventilator-associated pneumonia (VAP). This rapid detection of Gram-negative bacteria aids in optimizing patient treatment.

Area of Science:

  • Medical Microbiology
  • Infectious Diseases
  • Clinical Diagnostics

Background:

  • Late-onset ventilator-associated pneumonia (VAP) is a severe infection often caused by multidrug-resistant Gram-negative bacteria.
  • Empiric broad-spectrum antibiotic treatment is typically required for VAP, increasing the risk of resistance.
  • Rapid and accurate pathogen identification is crucial for effective VAP management.

Purpose of the Study:

  • To evaluate the BioFire FilmArray Blood Culture Identification (BCID) panel for rapid detection of VAP pathogens.
  • To assess the panel's performance in identifying common Gram-negative bacteria responsible for VAP.
  • To determine the clinical utility of the BCID panel in optimizing VAP treatment.

Main Methods:

  • An international multicentre clinical trial (MagicBullet) was conducted across 32 hospitals in Spain, Greece, and Italy.
  • Respiratory samples were collected from 165 patients with suspected VAP.
  • Microorganism identification was performed using the BCID panel and compared to conventional microbiologic techniques.

Main Results:

  • Pseudomonas aeruginosa, Acinetobacter baumannii, and Klebsiella pneumoniae were the most frequent pathogens (54.7%).
  • The BCID panel demonstrated high specificity (98.1%) and negative predictive value (96.6%).
  • Sensitivity was 78.6% and positive predictive value was 87.3% for these key microorganisms, with results available in 1 hour.

Conclusions:

  • The BCID panel shows clinical utility in rapidly ruling out VAP-causing microorganisms, particularly multidrug-resistant Gram-negative species.
  • Faster identification can facilitate the optimization of empiric antimicrobial therapy for VAP.
  • The panel's speed and accuracy support its use in managing VAP in intensive care settings.
Keywords:
Antibiotic resistanceFilmArray BCID panelGram-negative bacteriaRapid diagnosisVentilator-associated pneumonia

More Related Videos

Rapid Identification of Gram Negative Bacteria from Blood Culture Broth Using MALDI-TOF Mass Spectrometry13:29

Rapid Identification of Gram Negative Bacteria from Blood Culture Broth Using MALDI-TOF Mass Spectrometry

Published on: May 28, 2014

25.0K
A 1.5 Hour Procedure for Identification of Enterococcus Species Directly from Blood Cultures05:02

A 1.5 Hour Procedure for Identification of Enterococcus Species Directly from Blood Cultures

Published on: February 10, 2011

24.6K

Related Concept Videos

Methods of Classification and Identification01:28

Methods of Classification and Identification

Bacterial identification relies on a diverse array of techniques to classify and understand microorganisms, each tailored to uncover specific characteristics. Traditional morphological approaches, while still valuable, are limited for closely related or structurally simple organisms. Modern methods integrate biochemical, serological, genetic, and advanced molecular tools to achieve greater accuracy.Morphological and Biochemical TechniquesMorphological characteristics, such as cell shape and...
1.2K
Correlation and Causation01:27

Correlation and Causation

Statistical tests can calculate whether there is a relationship, or correlation, between independent and dependent variables. An indirect relationship of the variables signifies a correlation, while a direct relationship shows causation. If it is determined that no connection exists between the variables, then the correlation is a coincidence.
Correlation versus Causation
If the dependent variable increases or decreases when the independent variable increases, there is a positive or negative...
42.9K
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
8.5K
Local Anesthetics: Common Agents and Their Applications01:23

Local Anesthetics: Common Agents and Their Applications

Local anesthetics (LAs) are commonly used for various applications in medical and dental procedures. Some of the common agents used are cocaine, lidocaine, and bupivacaine.
Cocaine is an ester of benzoic acid and methylecgogine. It is used to anesthetize and vasoconstrict locally. Currently, it is used primarily for topical applications. It is beneficial for surgeries on the upper respiratory tract, providing anesthesia and shrinking the mucosa. Cocaine in the form of cocaine hydrochloride is...
981
Wood Panel Products01:18

Wood Panel Products

Wood panel products are essential materials used in construction for applications such as flooring, siding, and roofing, typically available in standard dimensions of 4 feet by 8 feet, with thicknesses varying from one-quarter of an inch to one and one-eighth inches. Among the most common types of wood panels is plywood, which is produced by gluing multiple layers of thin wood veneers under pressure. The grain of the outer veneers runs lengthwise, while the grains of the interior layers run...
400
Mechanical Ventilation II: Invasive Ventilation01:23

Mechanical Ventilation II: Invasive Ventilation

Ventilators are essential medical equipment used to aid patients with respiratory difficulties. Their primary function is to assist or replace spontaneous breathing by providing mechanical ventilation. There are two general classes of mechanical ventilators: negative-pressure and positive-pressure ventilators.
Negative-Pressure Ventilators
Negative-pressure ventilators create a vacuum around the chest or body to draw air into the lungs, simulating breathing. This method does not require an...
781