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Method for selecting optimal cells for enterovirus isolation as determined in an outbreak of echovirus type 33
European Journal of Clinical Microbiology
|June 1, 1985
Abstract:
An outbreak of echovirus type 33-induced meningitis which occurred in Belgium in 1982 is reported. To identify the causative agent, titers of an early isolate were measured on a variety of cells in order to select the optimal cells. A human rhabdomyosarcoma cell line was found to be the substrate of choice due to its proficient isolation of the virus. This method of determining infectious titres is recommended for improving enterovirus isolation in other epidemics.
Insights
An echovirus type 33 outbreak in Belgium caused meningitis. Researchers found a human rhabdomyosarcoma cell line optimal for isolating the virus, improving future enterovirus detection.
Area of Science:
- Virology
- Epidemiology
- Cell Biology
Background:
- A 1982 outbreak of meningitis in Belgium was linked to echovirus type 33.
- Enterovirus isolation is crucial for understanding and managing viral epidemics.
Purpose of the Study:
- To identify the optimal cell line for isolating echovirus type 33 during an epidemic.
- To establish a reliable method for improving enterovirus isolation in future outbreaks.
Main Methods:
- An early isolate of echovirus type 33 was used to measure viral titers.
- Titers were assessed across various cell types to determine the most effective substrate.
- A human rhabdomyosarcoma cell line was identified as the preferred cell line.
Main Results:
- The human rhabdomyosarcoma cell line demonstrated proficient isolation of echovirus type 33.
- This cell line proved superior to other tested substrates for viral recovery.
Conclusions:
- Human rhabdomyosarcoma cells are highly effective for echovirus type 33 isolation.
- The methodology for selecting optimal cell lines can enhance enterovirus isolation during epidemics.