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Author Spotlight: Surgical Methods and Outcomes in Oviductal Cloned Pig Embryo Transfers
Published on: October 18, 2024
Alpaca embryo transfer on a private Canadian farm
J Manuel Palomino1, Lori Jones1, Tom Vanhanen1
1Department of Biomedical Sciences, University of Guelph, Guelph, Ontario N1G 2W1 (Palomino); Arriba Linea alpacas, Uxbridge, Ontario (Jones, Vanhanen); Reproductive Physiology, Toronto Zoo, Ontario (Mastromonaco); Port Perry Veterinary Services, Port Perry, Ontario (Busato); Department of Veterinary Biomedical Sciences, University of Saskatchewan, Saskatoon, Saskatchewan (Adams, Palomino).
Abstract:
This study evaluated the feasibility of using an embryo transfer protocol in an alpaca farm in Canada. Alpaca donors and recipients were synchronized with 2 doses of gonadotrophin-releasing hormone (GnRH), 12 days apart. In donors (n = 5), superstimulation was induced with follicle stimulating hormone (FSH) given daily (40 mg) for 5 days beginning 2 days after the second GnRH treatment. Cloprostenol was given on the last day of FSH, the donors were bred 2 days later, embryos were collected 7 days after breeding. In recipients (n = 8), the second dose of GnRH was given the day before donor mating, and embryos were transferred on the day of donor collection. On average (± SEM), 5.2 ± 1.4 corpora lutea were detected and 2.5 ± 1.2 transferable embryos were collected in the donors. A mature corpus luteum was detected in 6/8 synchronized recipients and a single embryo was transferred to each. One recipient alpaca became pregnant and delivered a healthy baby 349 days after embryo transfer. This is the first report of successful embryo transfer in alpacas in Canada.
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