Related Experiment Video
Updated: Aug 17, 2026

Characterization of Membrane Transporters by Heterologous Expression in E. coli and Production of Membrane Vesicles
Published on: December 31, 2019
Use Endosidin2 to Study Plant Exocytosis and Vacuolar Trafficking
1Department of Botany and Pathology, Purdue University, 915 W. State St., West Lafayette, IN, 47907, USA.
Abstract:
Membrane trafficking is essential for multiple aspects of plant growth and development. Some small molecules have been widely used to study the mechanisms of membrane trafficking in plants. For example, short-term treatment with small molecules combined with live cell imaging has been shown to be very useful in understanding the dynamic processes of membrane trafficking. Small molecule Endosidin2 (ES2) has been found to target Exo70 protein and inhibit exocytosis and promote vacuolar trafficking in plants. Here we describe the method of using short-term ES2 treatment combined with live cell imaging to study plant exocytosis and vacuolar trafficking in Arabidopsis seedlings expressing a cargo protein PIN2:GFP.
Related Concept Videos
Receptor-mediated Endocytosis
Exocytosis
Receptor-mediated Endocytosis
Clathrin-Mediated Endocytosis of LDL
One well-characterized example of receptor-mediated endocytosis is the...
Exocytosis
Exocytosis is the opposite of endocytosis, which brings molecules inside the cell. Sometimes, the released materials are signaling molecules. For example, neurons typically use exocytosis to release neurotransmitters. Cells also use exocytosis to insert proteins such as ion channels into their cell membranes, secrete proteins for use in the extracellular matrix, or...
Overview of Secretory Vesicles
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
Vesicular Trasport: Endocytosis, Transcytosis and Exocytosis
Endocytosis is a cellular mechanism that involves the inward folding of the cell membrane to create vesicles that capture and transport large drug molecules. This process comprises two distinct methods: pinocytosis (often referred to as "cell drinking") and phagocytosis (often referred to as "cell eating"). Pinocytosis is...

