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Zebrafish Whole Mount High-Resolution Double Fluorescent In Situ Hybridization
Published on: March 25, 2009
Whole-Mount BrdU Staining with Fluorescence In Situ Hybridization in Planarians
Li-Chun Cheng1, Alejandro Sánchez Alvarado2
1The Scripps Research Institute, La Jolla, CA, USA.
Abstract:
Detection of cell proliferation based on the incorporation of 5'-bromo-2'-deoxyuridine (BrdU) has become a standard approach for studying stem cell and progenitor cell populations in developing and adult tissue. In this chapter, we describe three BrdU administration methods for planarians and a staining protocol combining BrdU detection with whole-mount fluorescence in situ hybridization (FISH). Collectively, these protocols enable the combined analysis of BrdU-incorporation and endogenous gene expression, as for example during lineage tracing applications.
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