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Related Experiment Videos

Yeast contains two functional genes coding for ribosomal protein S10.

R J Leer, M M van Raamsdonk-Duin, C M Molenaar

    Nucleic Acids Research
    |July 25, 1985
    PubMed
    Summary

    Researchers analyzed two yeast ribosomal protein S10 gene copies, finding identical coding regions but divergent non-coding sequences. Both genes are expressed, though their transcript levels vary, impacting protein production in yeast cells.

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    Modified sequences in yeast ribosomal RNA.

    Molecular biology reports·2013

    Area of Science:

    • Molecular Biology
    • Yeast Genetics
    • Gene Expression Analysis

    Background:

    • Ribosomal proteins are essential for protein synthesis in all organisms.
    • Yeast ribosomal protein S10 plays a crucial role in ribosome assembly and function.
    • Understanding gene copy variations is key to deciphering gene regulation.

    Purpose of the Study:

    • To determine and compare the DNA sequences of two gene copies encoding yeast ribosomal protein S10.
    • To identify the transcription start and termination sites for these genes.
    • To investigate the expression levels and regulation of these distinct gene copies in yeast.

    Main Methods:

    • DNA sequencing to analyze gene structures.
    • Northern blot or RT-PCR to analyze transcript levels.

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  • Sequence comparison to identify homologies and differences.

    • Main Results:

    • The two gene copies for yeast ribosomal protein S10 possess virtually identical amino acid coding regions.
    • Significant sequence differences were observed in the leader and trailer regions, with minimal homology in intervening sequences.
    • Both gene copies are actively transcribed in vegetatively growing yeast cells.
    • The relative amounts of transcripts from the two gene copies differ.

    Conclusions:

    • Despite sequence divergence in regulatory regions, both ribosomal protein S10 gene copies are functional and expressed in yeast.
    • Differential expression levels suggest a regulatory mechanism controlling the contribution of each gene copy to the total protein pool.
    • Sequence variations in non-coding regions likely play a role in modulating gene expression and transcript abundance.