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In Vivo Infection with Leishmania amazonensis to Evaluate Parasite Virulence in Mice
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In Vivo Infection with Leishmania amazonensis to Evaluate Parasite Virulence in Mice

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CD100/Sema4D Increases Macrophage Infection by Leishmania (Leishmania) amazonensis in a CD72 Dependent Manner

Mariana K Galuppo1, Eloiza de Rezende1, Fabio L Forti2

  • 1Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.

Frontiers in Microbiology
|June 21, 2018
PubMed

Insights

Soluble CD100 (sCD100) increases macrophage infection by Leishmania parasites. This effect is mediated by the CD72 receptor, highlighting sCD100 as a potential therapeutic target for leishmaniasis.

Area of Science:

  • Immunology
  • Parasitology
  • Cell Biology

Background:

  • Leishmaniasis is a significant global health concern caused by Leishmania protozoa, primarily infecting macrophages.
  • The disease presents in various forms (cutaneous, mucocutaneous, visceral), influenced by Leishmania species and host immunity.
  • CD100, a protein found on lymphocytes and induced in macrophages, exists in membrane-bound and soluble forms (sCD100).

Purpose of the Study:

  • To investigate CD100 expression in murine macrophages during Leishmania infection.
  • To determine the impact of soluble CD100 (sCD100) on macrophage susceptibility to Leishmania (L.) amazonensis infection.
  • To explore the role of the CD72 receptor in mediating sCD100 effects on macrophages.

Main Methods:

  • Analysis of CD100 expression in murine macrophages post-Leishmania infection.
  • In vitro experiments assessing the effect of sCD100 on macrophage phagocytosis of Leishmania promastigotes.
  • Evaluation of sCD100's influence on macrophage uptake of zymosan particles and Trypanosoma cruzi infection.

Main Results:

  • CD100 expression in murine macrophages significantly increases following Leishmania infection.
  • sCD100 enhances both the phagocytosis of Leishmania (L.) amazonensis promastigotes and subsequent macrophage infection.
  • This augmentation of infection by sCD100 is dependent on the presence of the CD72 receptor on macrophages.
  • sCD100 also boosts the phagocytosis of zymosan particles and enhances Trypanosoma cruzi infection in macrophages.

Conclusions:

  • Leishmania infection upregulates CD100 expression in macrophages.
  • sCD100 promotes Leishmania infection and parasite uptake by macrophages, mediated through the CD72 receptor.
  • These findings suggest sCD100 plays a role in modulating macrophage-parasite interactions and could be a target for therapeutic strategies against leishmaniasis and other parasitic infections.
Keywords:
CD100CD72Leishmania amazonensisinfection indexmacrophagesphagocytosis

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