Related Experiment Videos
A high-performance liquid chromatographic technique that separates cellular retinol binding protein from cellular
Analytical Biochemistry
|May 15, 1985
Abstract:
A one-step procedure to detect cellular [3H]retinol and [3H]retinoic acid binding proteins (CRBP and CRABP) from rat testis cytosolic extract was devised. The procedure is based on anion-exchange high-performance liquid chromatography of the cytosolic fraction on columns of Mono Q, which permits elution of CRABP and CRBP at 12 and 22 min, respectively.