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Author Spotlight: Analyzing Fibrosis Development in Chronic Lung Allograft Rejection Using Picrosirius Red Staining in Mouse Models
Published on: March 21, 2025
Evaluation of dermal collagen stained with picrosirius red and examined under polarized light microscopy
Polyana Galvão Bernardes Coelho1, Maria Verônica de Souza1, Lissandro Gonçalves Conceição1
1Department of Veterinary, Universidade Federal de Viçosa, Viçosa, MG, Brazil.
Abstract:
The special picrosirius red staining highlights the natural birefringence of collagen fibers when exposed to polarized light. The results from birefringence allow to evaluate the organization of the collagen fibers in the tissues. The authors intend to elucidate all steps to obtain and capture images of histological sections stained with picrosirius red and evaluated under polarized light microscopy, as well as possible artefacts that may occur.
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