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[The expressions and the roles of SDF-1/CXCR-4 and SDF-1/CXCR-4 in human endometriosis]

Z Ouyang¹, J P Sun, X L Tian

¹Department of Gynecology and Obstetrics, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China.

Zhonghua Yi Xue Za Zhi

|June 21, 2018

Abstract:

Objective: To investigate the expressions and significances of SDF-1 and its receptor CXCR-4 in endometriosis. Methods: 50 hysteromyoma patients treated at Beijing Tongren Hospital, Capital Medical University between 1(st) January 2016 and 31(st) December 2017 were divided into control group, that is, non-endometriosis group, while another 50 endometriosis patients were divided into experimental group.The endometrial tissues, endometriosis lesions, and peritoneal fluid samples of hysteromyoma patients were collected by operation.RT-PCR, ELISA and immunohistochemistry were adopted to detect the expressions of SDF-1 and CXCR-4 in the two groups.Cell count was used to analyze the roles of SDF-1 and CXCR4 in the mitosis and proliferation of endometrial stromal cells. Results: The mean value of SDF-1 and CXCR-4 expressions in ascites or peritoneal fluid of endometriosis patients were (2.56±0.33) mg/L and (4.47±0.32) mg/L, respectively. The mean concentrations in ascites or peritoneal fluid in hysteromyoma patients were (1.39±0.36) mg/L and (3.16±0.32) mg/L, respectively.The expressions of SDF-1 and CXCR-4 in ascites or peritoneal fluid of endometriosis patients were both significantly higher than those of patients in non-endometriosis group (P<0.05). SDF-1 and CXCR-4 were expressed in both endometriosis lesions and the glandular epithelial cells and mesenchymal cells of normal endometrial tissue.Positive staining sites were located in the cytoplasm.A value was used to calculate and analyze the expression of immune staining.The mean A value of SDF-1 and CXCR-4 in endometriosis group were 0.21±0.13 and 0.21±0.13, respectively, and the mean A value in normal endometrial tissues were 0.15±0.13 and 0.14±0.13, respectively.The expressions of these two in endometrial tissues were significantly higher than that in normal endometrial tissues, and the differences were statistically significant (P<0.05). The expression of CXCR-4 mRNA was abundant in the mesenchymal cells of endometriosis cultured in vitro.SDF-1 promoted the mitosis and proliferation of endometrial stromal cells cultured in vitro in a dose-dependent manner.The neutralizing antibody against CXCR-4 was obviously inhibited. Conclusion: The high expressions of SDF-1 and CXCR-4 in endometriosis as well as SDF-1 through its specific receptor CXCR-4 promoted the mitosis and proliferation of endometrial stromal cells, suggesting that SDF-1 and CXCR-4 played important roles in the pathogenesis of endometriosis.

Insights

High levels of Stromal cell-derived factor 1 (SDF-1) and its receptor CXCR-4 are found in endometriosis. These molecules promote endometrial stromal cell proliferation, indicating their significant role in the disease

Area of Science:

Reproductive medicine
Gynecology
Molecular biology

Background:

Endometriosis is a condition where endometrial-like tissue grows outside the uterus.
The molecular mechanisms underlying endometriosis pathogenesis are not fully understood.
Stromal cell-derived factor 1 (SDF-1) and its receptor CXCR-4 are implicated in various cellular processes.

Purpose of the Study:

To investigate the expression levels of SDF-1 and CXCR-4 in endometriosis.
To determine the significance of SDF-1 and CXCR-4 in the pathogenesis of endometriosis.
To explore the role of SDF-1 and CXCR-4 in endometrial stromal cell proliferation.

Main Methods:

Samples from 50 endometriosis patients and 50 non-endometriosis controls were analyzed.
RT-PCR, ELISA, and immunohistochemistry were used to detect SDF-1 and CXCR-4 expression.
In vitro cell culture and cell count assays assessed the impact of SDF-1 and CXCR-4 on endometrial stromal cells.

Main Results:

SDF-1 and CXCR-4 expression levels were significantly higher in peritoneal fluid and tissues of endometriosis patients compared to controls.
Both SDF-1 and CXCR-4 were expressed in endometriosis lesions and normal endometrial tissues.
SDF-1 promoted endometrial stromal cell mitosis and proliferation in a dose-dependent manner, inhibited by CXCR-4 neutralizing antibody.

Conclusions:

Elevated SDF-1 and CXCR-4 expression is characteristic of endometriosis.
SDF-1, via its receptor CXCR-4, significantly promotes endometrial stromal cell proliferation.
SDF-1 and CXCR-4 play crucial roles in the pathogenesis of endometriosis.

Read full analysis

Keywords:

CXCR-4 Endometriosis SDF-1

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