Spherical and Spindle-Like Abamectin-Loaded Nanoparticles by Flash Nanoprecipitation for Southern Root-Knot Nematode...

Southern root-knot nematode (Meloidogyne incognita) is a biotrophic parasite, causing enormous loss in global crop production annually. Abamectin (Abm) is a biological and high-efficiency pesticide against Meloidogyne incognita. In this study, a powerful method, flash nanoprecipitation (FNP), was adopted to successfully produce Abm-loaded nanoparticle suspensions with high drug loading capacity (>40%) and encapsulation efficiency (>95%), where amphiphilic block copolymers (BCPs) poly(lactic-co-glycolic acid)-b-poly(ethylene glycol) (PLGA-b-PEG), poly(d,l-lactide)-b-poly(ethylene glycol) (PLA-b-PEG), or poly(caprolactone)-b-poly(ethylene glycol) (PCL-b-PEG) were used as the stabilizer to prevent the nanoparticles from aggregation. The effect of the drug-to-stabilizer feed ratio on the particle stability were investigated. Moreover, the effect of the BCP composition on the morphology of Abm-loaded nanoparticles for controlling Meloidogyne incognita were discussed. Notably, spindle-like nanoparticles were obtained with PCL-b-PEG as the stabilizer and found significantly more efficient (98.4% mortality at 1 ppm particle concentration) than spherical nanoparticles using PLGA-b-PEG or PLA-b-PEG as the stabilizer. This work provides a more rapid and powerful method to prepare stable Abm-loaded nanoparticles with tunable morphologies and improved effectiveness for controlling Meloidogyne incognita.