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An Organotypic High Throughput System for Characterization of Drug Sensitivity of Primary Multiple Myeloma Cells
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[Effects of triptolide on the apoptosis and H3K4 protein methylation in multiple myeloma cells]

Cheng-Bo Xu1, Bin Liao1, Jian-Zhen Shen2

  • 1Department of Hematology, The People's Hospital Affiliated to Fujian University of Traditional Chinese Medicine, Fuzhou 350004.

Zhongguo Ying Yong Sheng Li Xue Za Zhi = Zhongguo Yingyong Shenglixue Zazhi = Chinese Journal of Applied Physiology
|June 22, 2018
PubMed
Abstract

Insights

Triptolide, a traditional Chinese medicine, inhibits multiple myeloma cell growth and induces apoptosis. It also reduces H3K4 methylation by altering SMYD3 and LSD1 expression, suggesting an anti-cancer mechanism.

Area of Science:

  • Oncology
  • Epigenetics
  • Pharmacology

Background:

  • Multiple myeloma (MM) is a hematological malignancy.
  • Understanding novel therapeutic targets is crucial for MM treatment.
  • Epigenetic modifications play a role in cancer development.

Purpose of the Study:

  • To investigate the effects of triptolide on multiple myeloma cell apoptosis.
  • To examine the impact of triptolide on H3K4 protein methylation in MM cells.
  • To explore the underlying molecular mechanisms of triptolide's anti-cancer activity.

Main Methods:

  • RPMI8226 multiple myeloma cells were treated with varying concentrations of triptolide.
  • Cell proliferation was assessed using MTT assay.
  • Apoptosis, cell cycle distribution, H3K4me2, H3K4me3, SMYD3, and LSD1 expression were analyzed via flow cytometry and Western blot, qRT-PCR.

Main Results:

  • Triptolide significantly inhibited RPMI8226 cell proliferation in a dose- and time-dependent manner.
  • Triptolide induced apoptosis and G2/M cell cycle arrest.
  • Triptolide decreased H3K4me2 and H3K4me3 levels, downregulated SMYD3, and upregulated LSD1.

Conclusions:

  • Triptolide exhibits anti-proliferative and pro-apoptotic effects on multiple myeloma cells.
  • Triptolide alters H3K4 methylation by modulating SMYD3 and LSD1 expression.
  • These epigenetic changes likely contribute to triptolide's anti-cancer mechanism in multiple myeloma.
Keywords:
apoptosishistone proteinmethylationmultiple myelomatriptolide

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