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Nutrient Regulation by Continuous Feeding for Large-scale Expansion of Mammalian Cells in Spheroids
Published on: September 25, 2016
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Eisosomes are metabolically regulated storage compartments for APC-type nutrient transporters
Akshay Moharir1, Lincoln Gay1, Daniel Appadurai1
1Henry Eyring Center for Cell and Genome Science, University of Utah, Salt Lake City, UT 84112.
Molecular Biology of the Cell
|June 22, 2018
Summary
Yeast eisosomes, similar to caveolae, store and inactivate nutrient transporters (APC). They maintain the proton gradient by balancing transporters with proton pumps, restructuring when the gradient is disrupted.
Area of Science:
- Cell biology
- Membrane biophysics
- Yeast genetics
Background:
- Eisosomes are yeast plasma membrane lipid domains.
- They share similarities with higher eukaryotic caveolae.
- The role of eisosomes in harboring APC-type nutrient transporters is unclear.
Purpose of the Study:
- To investigate the function of eisosomes in yeast.
- To understand the relationship between eisosomes and APC nutrient transporters.
- To elucidate the role of eisosomes in maintaining plasma membrane homeostasis.
Main Methods:
- Analysis of yeast plasma membrane structure and function.
- Investigating the interaction between eisosomes and APC transporters.
- Studying the impact of proton gradient disruption on eisosome dynamics.
Main Results:
- Eisosomes act as storage compartments for APC transporters, maintaining them in a stable, inactive state.
- Yeast regulates eisosomes to balance APC transporters with proton pump activity (Pma1), preserving the proton gradient.
- Disruption of the proton gradient triggers rapid eisosome restructuring and APC transporter removal from the cell surface.
- Eisosomes require APC transporters, indicating their role in regulating transporter activity.
Conclusions:
- Eisosomes are crucial for regulating yeast nutrient uptake by controlling APC transporter localization and activity.
- Eisosome dynamics are directly linked to the plasma membrane proton gradient, highlighting their role in cellular homeostasis.
- The findings suggest that eisosomes function as a key regulatory platform for nutrient transport in yeast.
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