Related Experiment Video
Updated: Jun 12, 2025

Three-dimensional Characterization of Interorganelle Contact Sites in Hepatocytes using Serial Section Electron Microscopy
Published on: June 9, 2022
ER-plasma membrane contact sites deliver ER lipids and proteins for rapid cell surface expansion
Madison Smith1, Lincoln Gay1, Markus Babst1
1Henry Eyring Center for Cell and Genome Science, University of Utah , Salt Lake City, UT, USA.
Abstract:
As a consequence of hypoosmotic shock, yeast cells swell rapidly and increase the surface area by ∼20% in 20 s. Approximately, 35% of this surface increase is mediated by the ER-plasma membrane contact sites, specifically the tricalbins, which are required for the delivery of both lipids and the GPI-anchored protein Crh2 from the cortical ER to the plasma membrane. Therefore, we propose a new function for the tricalbins: mediating the fusion of the ER to the plasma membrane at contact sites. This proposed fusion is triggered by calcium influx via the stretch-gated channel Cch1 and is supported by the anoctamin Ist2.
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