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Updated: Feb 8, 2026

Structural Characterization of Mannan Cell Wall Polysaccharides in Plants Using PACE
Published on: October 16, 2017
Structures of O-specific polysaccharides of Pseudomonas psyhrotolerans BIM B-1158G
Evelina L Zdorovenko1, Alexander S Shashkov1, Alexandra A Kadykova1
1N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991, Moscow, Russian Federation.
Abstract:
Strain of Pseudomonas psychrotolerans was cultured on the nutrient agar and in a liquid nutrient broth. Bacterial cells were phage-typed with bacteriophages specific to Pseudomonas. O-antigen was isolated from cells using phenol-water method and mild acid degradation. The following structures of the polysaccharides extracted were established by sugar analysis and 1D, 2D NMR spectroscopy: PSI→3)-α-D-Manp-(1→2)-α-D-Manp-(1→; PSII→3)-α-D-Rhap-(1→2)-β-D-Rhap-(1→3)-α-D-Rhap-(1→; α-D-Glcp-(1˩; 2.
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