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Complete Genome Sequences of HonestAbe, Anthony, and Taffo16, Three Cluster C Bacillus cereus Group Bacteriophages

Martin Lee1, Kayla M Puglisi1,

  • 1Department of Biological Sciences, University of Maryland, Baltimore County, Baltimore, Maryland, USA.

Genome Announcements
|June 23, 2018
PubMed

Insights

Three novel bacteriophages infecting Bacillus cereus group bacteria were identified. These cluster C Myoviridae phages, isolated from soil, show high genetic similarity within their groups, aiding in bacterial research.

Area of Science:

  • Microbiology
  • Virology
  • Genomics

Background:

  • The Bacillus cereus group comprises significant human and animal pathogens.
  • Bacteriophages, viruses that infect bacteria, are crucial in microbial ecology and potential therapeutic agents.
  • Characterizing novel phages provides insights into bacterial-viral interactions and phage diversity.

Purpose of the Study:

  • To isolate and characterize novel bacteriophages targeting Bacillus cereus group bacteria.
  • To determine the phylogenetic placement of newly isolated phages within the Myoviridae family.
  • To assess the genetic relatedness of these phages using whole-genome sequence data.

Main Methods:

  • Bacteriophage isolation from soil samples using enrichment culture techniques.
  • Host specificity assays using Bacillus thuringiensis subsp. kurstaki.
  • Genomic DNA extraction, sequencing, and comparative analysis of isolated phages.

Main Results:

  • Isolation of three distinct cluster C Myoviridae bacteriophages: HonestAbe, Anthony, and Taffo16.
  • Phages were confirmed to infect bacteria within the Bacillus cereus group.
  • Average nucleotide identities among the isolated phages exceeded 90% within their respective subclusters.

Conclusions:

  • The identified bacteriophages represent new additions to the Myoviridae family infecting Bacillus cereus group bacteria.
  • High nucleotide identity suggests potential for phage-based biocontrol strategies and further genomic studies.
  • This discovery expands the known diversity of bacteriophages targeting important bacterial pathogens.

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