Related Experiment Video

Updated: Feb 8, 2026

Mechanism of Kemeng Fang's Inhibition of Podocyte Apoptosis in Rats with Membranous Nephropathy through the PI3K/AKT Signaling Pathway
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Mechanism of Kemeng Fang's Inhibition of Podocyte Apoptosis in Rats with Membranous Nephropathy through the PI3K/AKT Signaling Pathway

Published on: August 23, 2024

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Isoliquiritigenin Suppresses Osteosarcoma U2OS Cell Proliferation and Invasion by Regulating the PI3K/Akt Signalling

Jing Chen1, Cheng Liu2, Qin-Qing Yang3

  • 1Department of Orthopaedics, the Second Hospital of Peking Armed Polce, Beijing, China.

Chemotherapy
|June 25, 2018
PubMed
Abstract

Insights

Isoliquiritigenin (ISL) effectively inhibits osteosarcoma cell growth and promotes apoptosis. This flavonoid may serve as a potential therapeutic agent for osteosarcoma by suppressing the PI3K/Akt signaling pathway.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Oncology

Background:

  • Isoliquiritigenin (ISL) is a flavonoid with known antioxidant, vasorelaxant, anti-inflammatory, and antitumor properties.
  • Osteosarcoma is a primary bone cancer with significant treatment challenges.

Purpose of the Study:

  • To investigate the antitumor effects of ISL on human osteosarcoma U2OS cells.
  • To elucidate the underlying molecular mechanisms of ISL's action in osteosarcoma.

Main Methods:

  • Cell proliferation, invasion, and migration assays (CCK8, Transwell).
  • Apoptosis analysis using fluorescence-activated cell sorting.
  • Western blotting to assess protein expression (Bcl-2, Bax, Caspase-3, Akt, mTOR, Cyclin D1).

Main Results:

  • ISL significantly suppressed U2OS cell proliferation, invasion, and migration.
  • ISL treatment promoted apoptosis, evidenced by increased Bax and active Caspase-3, and decreased Bcl-2.
  • ISL reduced phosphorylation of Akt and mTOR, indicating PI3K/Akt pathway inhibition.

Conclusions:

  • ISL exhibits potential as a therapeutic agent for osteosarcoma.
  • ISL may exert its antitumor effects by suppressing the PI3K/Akt signaling pathway.
  • Further research into ISL for osteosarcoma treatment is warranted.
Keywords:
ApoptosisInvasionIsoliquiritigeninOsteosarcomaPI3K/Akt signalling pathwayProliferation

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