Related Experiment Video
Updated: Feb 8, 2026
09:20
Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues
Published on: March 31, 2016
30.1K
Comparing stemness gene expression between stem cell subpopulations from peripheral blood and adipose tissue
Maria Teresa González-Garza1, Delia E Cruz-Vega1, Alejandro Cárdenas-Lopez2
1Tecnologico de Monterrey, Escuela de Medicina y Ciencias de la Salud Morones Prieto 3000 Pte. Monterrey NL. CP64710, México.
American Journal of Stem Cells
|June 26, 2018
Summary
This study compared stemness gene expression in peripheral blood and adipose tissue stem cells. Findings reveal distinct gene expression patterns, guiding optimal cell therapy applications for specific tissue regeneration.
Area of Science:
- Regenerative Medicine
- Stem Cell Biology
- Cell Therapy
Background:
- Cell therapy offers promise for degenerative diseases, utilizing adult stem cells from bone marrow, adipose tissue, and peripheral blood.
- Identifying specific stem cell subpopulations with optimal differentiation potential remains a challenge due to insufficient comparative data.
- Understanding stemness gene expression is crucial for selecting appropriate cell types for targeted therapeutic applications.
Purpose of the Study:
- To analyze and compare stemness gene expression in plastic-adherent cells from peripheral blood and adipose tissue.
- To investigate subpopulations selected by CD133+ and CD271+ markers for their self-renewal capacity and gene expression profiles.
- To evaluate the potential of different stem cell subtypes for specific tissue differentiation in cell therapy.
Main Methods:
- Isolation and culture of plastic-adherent stem cells from peripheral blood and adipose tissue.
- Immune selection of CD133+ and CD271+ subpopulations.
- Analysis of self-renewal capacity, surface markers (CD73, CD90, CD105, CD45) via flow cytometry, and stemness gene expression (NANOG, OCT4, SOX2, REX1, NOTCH1, NESTIN) via qPCR.
Main Results:
- All analyzed cell subpopulations met minimal criteria for human stem cells and demonstrated self-renewal capacity.
- CD133+ cells exhibited slower doubling times compared to other subpopulations.
- Significant differences in the quantity and pattern of stemness gene expression were observed among the subpopulations, suggesting distinct differentiation potentials.
Conclusions:
- Distinct stemness gene expression profiles in peripheral blood and adipose tissue stem cells, including CD133+ and CD271+ subpopulations, offer potential advantages for specific cell therapy applications.
- High OCT4 expression in CD133+ blood cells suggests potential for neuron differentiation, while low NOTCH1 in CD271+ cells may favor myoblast differentiation.
- Further experiments focusing on specific differentiation responses are warranted to confirm the therapeutic potential of these distinct stem cell subtypes.
Keywords:
ADSC-CD133+ADSC-CD271+ADSCsBlood mesenchymal stem cellsMSCsNESTINadipose derived stem cellsblood CD133+ cellsblood CD271+ cellsstemness genesMore Related Videos
09:45Generation of Integration-free Induced Pluripotent Stem Cells from Human Peripheral Blood Mononuclear Cells Using Episomal Vectors
Published on: January 1, 2017
11.4K
08:09Isolation of Rat Adipose Tissue Mesenchymal Stem Cells for Differentiation into Insulin-producing Cells
Published on: August 29, 2022
8.5K
Related Concept Videos
01:13Induced Pluripotent Stem Cells
28.1K
Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
28.1K
01:33Adult Stem Cells
33.9K
Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously...
33.9K
00:58Embryonic Stem Cells
32.5K
Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
32.5K
01:58Cell Specific Gene Expression
16.6K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
16.6K
01:58Cell Specific Gene Expression
5.6K
5.6K
01:23Tissue Renewal without Stem Cells
2.1K
After cellular or tissue damage, the resident stem cells present in the human body can locally repair and regenerate the damaged tissue or organ. However, even though some tissues do not have stem cells, they can repair and regenerate with the help of pre-existing cells. For example, beta cells of the pancreas and hepatocytes of the liver can divide to renew and regenerate the tissue. Here, both cell division and cell death are well regulated by homeostasis.
However, failure of such a system...
However, failure of such a system...
2.1K