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Related Experiment Video

Updated: Feb 8, 2026

Generation of Human Motor Units with Functional Neuromuscular Junctions in Microfluidic Devices
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Functional cargo delivery into mouse and human fibroblasts using a versatile microfluidic device.

Kevin H Lam1, Antonio Fernandez-Perez2, David W Schmidtke1

  • 1Department of Bioengineering, University of Texas at Dallas, Richardson, TX, USA.

Biomedical Microdevices
|June 26, 2018
PubMed
Summary

A new Polydimethylsiloxane (PDMS) microfluidic device, Cyto-PDMS, enables efficient intracellular cargo delivery for cell therapies. This versatile system optimizes delivery of various cargo sizes with minimal impact on cell viability.

Keywords:
Actin networkDrug deliveryIntracellular cargo deliveryPDMS

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Area of Science:

  • Biotechnology
  • Cell Biology
  • Bioengineering

Background:

  • Efficient intracellular delivery of therapeutic molecules is crucial for advancing stem cell and reprogramming therapies.
  • Existing silicon-based microfluidic devices face scalability limitations due to application-specific optimization requirements for shear-induced pore formation.

Purpose of the Study:

  • To develop a low-cost, versatile microfluidic device for efficient and optimized intracellular cargo delivery.
  • To create a system facilitating rapid prototyping for diverse academic research applications.

Main Methods:

  • Design and implementation of a Polydimethylsiloxane (PDMS)-based microfluidic device named Cyto-PDMS.
  • Systematic workflow utilizing Cyto-PDMS to assess intracellular delivery of cargo ranging from 1-70 kDa.
  • Evaluation of cellular viability post-delivery and demonstration of biological applications.

Main Results:

  • Cyto-PDMS successfully achieved intracellular cargo delivery across a wide range of molecular sizes (1-70 kDa) with minimal impact on cell viability.
  • Demonstrated F-actin labeling in live human fibroblasts, showcasing Cyto-PDMS's utility for live-cell imaging.
  • Successfully delivered active recombinant Cre protein for genomic recombination in recipient fibroblasts, proving nuclear cargo delivery capability.

Conclusions:

  • Cyto-PDMS provides a cost-effective and versatile platform for intracellular delivery of small molecules to the cytoplasm and active cargo to the nucleus.
  • The PDMS-based system's adaptability holds potential for optimizing delivery across various cell types, broadening the impact of cellular therapies.