Related Experiment Video
Updated: Feb 8, 2026

Isolation and Profiling of MicroRNA-containing Exosomes from Human Bile
Published on: June 13, 2016
Definitive profiling of plasma bile acids as potential biomarkers for human liver diseases using UPLC-HRMS
Yao Shi1, Yang Gao1, Michael Van Parys1
1Bioanalytical Department, Covance Laboratories, Inc., 3301 Kinsman Blvd, Madison, WI 53704, USA.
Aim:
Investigation of bile acids (BAs) as biomarkers for liver and kidney diseases has gained momentum recently to fulfill the needs in drug development and clinical practice, but a thorough and rapid profiling of BAs in human plasma has been hindered by the large interindividual variability and lack of selective methods.
Results:
A selective and efficient UPLC-high resolution mass spectrometry method was developed and fully validated for the definitive profiling of 26 BAs in human plasma with a curve rage of 1-1000 ng/ml and a runtime of 7.2 min.
Conclusion:
Four BA combinations with good sensitivity and specificity show potential biomarker applications for liver injury and diseases.
More Related Videos
08:42Using Multi-fluorinated Bile Acids and In Vivo Magnetic Resonance Imaging to Measure Bile Acid Transport
Published on: November 27, 2016
11:00Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS
Published on: May 20, 2013
Related Concept Videos
Diseases of the Liver and Gallbladder
Cirrhosis is characterized by the scarring of hepatic lobules in the liver, which are replaced by fibrous tissue, affecting the liver's normal functioning. NAFLD, on the other hand, is caused by an excessive build-up of fat in the liver, not...
Acid–Base Equilibria: Activity-Based Definition of pH
In solutions of very low ionic strength—for example, pure water—the...
Bile
Bile is released when dietary fats enter...
High-Resolution Mass Spectrometry (HRMS)
Definite Integral
Definition of z-Transform