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Defence cascade in Verticillium-infected grafted tomato.

Ross N Nazar1, Xin Xu1, Josefa Blaya Fernandez1

  • 1a Department of Molecular and Cellular Biology , University of Guelph , Guelph , ON Canada.

Plant Signaling & Behavior
|June 26, 2018
PubMed
Summary

The tomato Ve1-gene confers resistance to Verticillium wilt by modulating cell-specific defense proteins. However, this resistance effect is not systemic, as Ve1 function is not transferred to susceptible tissues in grafted plants.

Keywords:
Ve R-locusVe1-geneVerticillium wiltdefence genesproteometomato

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Area of Science:

  • Plant Pathology
  • Molecular Plant Breeding
  • Proteomics

Background:

  • Tomato plants possess the Ve1-gene, which confers resistance to the vascular wilt pathogen Verticillium dahliae race 1.
  • Susceptible tomato varieties (ve1 plants) lack this resistance and are prone to Verticillium wilt.

Purpose of the Study:

  • To investigate the proteomic changes associated with Verticillium wilt in tomato.
  • To determine the specific effect of the Ve1-gene on the defense/stress protein cascade during infection.
  • To assess whether Ve1-gene function can be systemically transferred in grafted plants.

Main Methods:

  • Utilized reciprocal grafting of resistant and susceptible tomato near-isolines.
  • Employed label-free Liquid Chromatography-Mass Spectrometry (LC-MS) for proteomic analysis.
  • Quantified fungal colonization to correlate with defense responses.

Main Results:

  • Defense/stress protein responses are cell-specific and correlate with the level of fungal colonization.
  • The Ve1-gene mitigates the defense response in resistant tissues.
  • Ve1-gene-mediated resistance effects were not observed in susceptible tissues of grafted plants.

Conclusions:

  • The Ve1-gene's resistance mechanism against Verticillium wilt is localized and cell-specific.
  • Resistance is not transmissible to susceptible tissues through grafting.
  • Understanding Ve1 gene function is crucial for developing resistant tomato cultivars.