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Microfluidic Device for Recreating a Tumor Microenvironment in Vitro
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Vascularized microfluidic platforms to mimic the tumor microenvironment.

Rhys Michna1, Manasa Gadde2, Alican Ozkan1

  • 1Department of Mechanical Engineering, The University of Texas at Austin, Austin, Texas.

Biotechnology and Bioengineering
|June 26, 2018
PubMed
Summary

Advanced microfluidic platforms mimic the tumor microenvironment, enabling studies on cell interactions and particle transport in vascularized cancer models. These systems offer a better alternative to traditional animal and 2D models.

Keywords:
3D in vitro tumorendothelial permeabilitymicrofluidic tumor platformsparticle transportvascular vessels

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Area of Science:

  • Biomedical Engineering
  • Cancer Research
  • Microfluidics

Background:

  • Traditional in vivo animal and in vitro 2D models present challenges in accurately replicating the tumor microenvironment.
  • Microfluidic technology offers a promising avenue for developing more physiologically relevant in vitro tumor models.
  • Understanding cell-cell interactions within the tumor vasculature is crucial for comprehending cancer progression and treatment efficacy.

Purpose of the Study:

  • To design and fabricate advanced microfluidic platforms that mimic the vascularized tumor microenvironment.
  • To investigate the influence of endothelial-tumor cell interactions on transport phenomena within a hyperpermeable tumor vasculature.
  • To demonstrate the versatility of microfluidic systems in replicating complex tumor microvasculature geometries and studying particle transport.

Main Methods:

  • Development of novel microfluidic platforms using subtractive and additive tissue engineering techniques.
  • Fabrication of single endothelialized microchannels within collagen matrices for breast cancer cell studies.
  • Creation of complex, interconnected microfluidic networks mimicking highly vascularized tumor systems.
  • Utilizing confocal imaging to assess vessel leakiness and perfusing networks with fluorescent particles to study transport.

Main Results:

  • Demonstrated enhanced vessel leakiness in microfluidic platforms, recapitulating key features of the tumor microenvironment.
  • Showcased the impact of tumor-endothelial interactions on particle transport through the vasculature.
  • Successfully created and perfused complex microfluidic vascular networks, including those patterned from in vivo data.
  • Validated the ability of these platforms to replicate intricate tumor microvasculature geometries.

Conclusions:

  • Microfluidic vascular tumor platforms of varying complexity can be successfully developed.
  • These platforms are effective tools for studying spatial particle transport and cellular interactions within a simulated tumor microenvironment.
  • The developed systems offer a powerful, physiologically representative alternative for cancer research.