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Reaction-Based Color-Convertible Fluorescent Probe for Ferroptosis Identification
Leilei Shi1, Qinghua Guan1, Xihui Gao1
1School of Chemistry and Chemical Engineering , State Key Laboratory of Metal Matrix Composites, Shanghai Jiao Tong University , 800 Dongchuan Road , Shanghai 200240 , China.
Analytical Chemistry
|June 27, 2018
Summary
Researchers developed Quinos-4 (QS-4), a fluorescent probe for detecting ferroptosis. This probe rapidly identifies ferroptosis by changing color from red to green in living cells and tissues, aiding disease research.
Area of Science:
- Biochemistry
- Cell Biology
- Chemical Biology
Background:
- Ferroptosis is an iron-dependent cell death pathway crucial in various pathophysiological processes.
- Current methods lack specificity and speed for real-time ferroptosis monitoring in living systems.
- Reactive oxygen species (ROS) and oxygenases are key indicators of ferroptosis.
Purpose of the Study:
- To design and validate a novel fluorescent probe for specific and rapid detection of ferroptosis.
- To enable real-time monitoring of ferroptosis in both in vitro and in vivo settings.
Main Methods:
- Development of a quinoxalinone-based fluorescent probe (Quinos-4, QS-4) with a reactive thioether moiety.
- Investigating the probe's response to ferroptosis biomarkers like ROS and hemeoxygenase-1 (HO-1).
- Observing the fluorescence emission shift from red to green upon probe oxidation.
Main Results:
- QS-4 is oxidized to QSO-4 in the presence of high ROS and HO-1 levels, characteristic of ferroptosis.
- The probe exhibits a distinct color conversion from red to green fluorescence.
- This color change allows for sensitive and specific identification of ferroptosis.
Conclusions:
- The developed QS-4 probe provides a novel tool for rapid and specific ferroptosis detection.
- Its ability to monitor ferroptosis in vitro and in vivo opens new avenues for research and diagnostics.
- This probe facilitates a deeper understanding of ferroptosis's role in disease.
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