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Surfaces of revolution are formed when a two-dimensional curve is rotated around an axis, producing a three-dimensional shape. This concept is used in engineering tasks like determining the surface area of a rocket nozzle, where precise calculations are critical for applying uniform heat-resistant coatings. When a curve is revolved about the x-axis, it sweeps out a continuous surface whose area must be calculated accurately to estimate material requirements.Approximating with Conical BandsTo...
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Amplicon Sequencing using the Long-Read Sequencing Technologies
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The Third Revolution in Sequencing Technology.

Erwin L van Dijk1, Yan Jaszczyszyn1, Delphine Naquin1

  • 1Institute for Integrative Biology of the Cell, UMR9198, CNRS CEA Université Paris-Sud, Université Paris-Saclay, 9198 Gif sur Yvette Cedex, France.

Trends in Genetics : TIG
|June 27, 2018
PubMed
Summary
This summary is machine-generated.

The third revolution in sequencing technology is here with long-read methods, offering higher quality genome assemblies and direct epigenetic modification detection. These advanced techniques overcome the limitations of older sequencing technologies.

Keywords:
long-read sequencingnanopore sequencingnext-generation sequencingsingle-molecule real-time sequencingsynthetic long-read sequencingthird-generation sequencing

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Area of Science:

  • Genomics
  • Molecular Biology
  • Bioinformatics

Background:

  • Sanger sequencing revolutionized genomics 40 years ago.
  • Next-generation sequencing (NGS) significantly reduced costs and increased speed but has short-read limitations.
  • Third-generation/long-read sequencing technologies represent a major advancement.

Purpose of the Study:

  • To review and compare various long-read sequencing methods.
  • To discuss the applications, strengths, and weaknesses of these technologies.
  • To provide future perspectives on long-read sequencing.

Main Methods:

  • Review of current third-generation/long-read sequencing technologies.
  • Comparative analysis of different long-read platforms.
  • Discussion of applications in genomics and epigenetics.

Main Results:

  • Long-read methods enable high-quality genome assemblies.
  • These technologies allow direct detection of epigenetic modifications on native DNA.
  • Whole-transcript sequencing without assembly is possible with long-read methods.

Conclusions:

  • Long-read sequencing marks the third revolution in the field.
  • These methods offer significant advantages over previous technologies.
  • Future applications are vast, impacting genomics, epigenetics, and transcriptomics.