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Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor
Published on: February 16, 2018
Label-free fluorescent aptasensor berberine-based strategy for ultrasensitive detection of Hg2+ ion
Xiuli Song1, Baochun Fu2, Yifeng Lan3
1Department of Chemistry, Taiyuan Normal University, Jinzhong 030619, PR China; Institute of Environmental Science, Shanxi University, Taiyuan 030006, PR China.
Abstract:
A label-free fluorescent aptasensing platform was fabricated and a simple and rapid method to detect Hg2+ ion in aqueous solution was put forward by means of berberine and Hg2+ ion-aptamer are as the fluorescence probe and the recognition element, respectively. Various factors including the concentration of berberine, Hg2+ ion and Hg2+ ion-aptamer, pH effect and the reaction time were investigated in detail. Under the optimal experimental conditions, in the sensing system, the fluorescence intensity changes displayed a calibration response for Hg2+ ion in the range of 0.1 μM to 10.0 μM and the detection limit was of 7.7 nM (S/N = 3). The fabricated label-free fluorescence aptasensor is not only conveniently but also effectively applicable used for analysis of Hg2+ ion in blood serum and tap water samples and the recovery range is of 96.0%-105.7%. In brief, this study offers an easy, economical and stable assay system for detecting Hg2+ ion in rough condition.
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