Related Experiment Video
Updated: Feb 8, 2026
12:59
A Study of the Complexation of MercuryII with Dicysteinyl Tetrapeptides by Electrospray Ionization Mass Spectrometry
Published on: January 8, 2016
8.4K
Coaxial Electrospray Ionization for the Study of Rapid In-source Chemistry
Brynn N Sundberg1, Anthony F Lagalante2
1Department of Chemistry, Villanova University, 800 Lancaster Avenue, Villanova, PA, 19085, USA.
Journal of the American Society for Mass Spectrometry
|June 28, 2018
Summary
This study introduces a modified coaxial electrospray probe for studying rapid in-source chemistry. The novel dual-emitter design enables fast mixing and reaction manipulation for diverse applications.
Area of Science:
- Analytical Chemistry
- Mass Spectrometry
Background:
- Coaxial electrospray is established for dual-emitter applications.
- Its use for rapid in-source chemistry remains unexplored.
Purpose of the Study:
- To fabricate a micro-volume, dual-emitter coaxial electrospray probe.
- To investigate its utility for rapid in-source chemistry.
Main Methods:
- Modification of a standard electrospray probe to create a coaxial, micro-volume dual-emitter.
- Demonstration using relative ionization in biphasic electrospray, hydrogen-deuterium exchange, and protein supercharging.
Main Results:
- The modified probe facilitates rapid mixing within the Taylor cone.
- Effective manipulation of fast reactions with various solvents and analytes was achieved.
- Demonstrated low-cost potential for diverse applications.
Conclusions:
- The fabricated coaxial dual-emitter is suitable for rapid in-source chemistry.
- This approach offers a versatile and cost-effective platform for mass spectrometry applications.
Related Concept Videos
01:12Electrospray Ionization (ESI) Mass Spectrometry
2.4K
Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
2.4K
03:12Ionization Energy
43.5K
The amount of energy required to remove the most loosely bound electron from a gaseous atom in its ground state is called its first ionization energy (IE1). The first ionization energy for an element, X, is the energy required to form a cation with 1+ charge:
43.5K
02:58Chemistry of the Cell
48.1K
The cell is chemically composed of water, organic molecules and inorganic ions.
Water
The polarity of the water molecule and its resulting hydrogen bonding makes water a unique substance with special properties that are intimately tied to the processes of life. Life originally evolved in an aqueous environment, and most of an organism’s cellular chemistry and metabolism occur inside the aqueous contents of the cell’s cytoplasm. Special properties of water are its high heat capacity...
Water
The polarity of the water molecule and its resulting hydrogen bonding makes water a unique substance with special properties that are intimately tied to the processes of life. Life originally evolved in an aqueous environment, and most of an organism’s cellular chemistry and metabolism occur inside the aqueous contents of the cell’s cytoplasm. Special properties of water are its high heat capacity...
48.1K
02:58Chemistry of the Cell
9.6K
9.6K
01:31Energy Stored In A Coaxial Cable
2.1K
A coaxial cable consists of a central copper conductor used for transmitting signals, followed by an insulator shield, a metallic braided mesh that prevents signal interference, and a plastic layer that encases the entire assembly.
In the simplest form, a coaxial cable can be represented by two long hollow concentric cylinders in which the current flows in opposite directions. The magnetic field inside and outside the coaxial cable is determined by using Ampère's law. The magnetic field inside...
In the simplest form, a coaxial cable can be represented by two long hollow concentric cylinders in which the current flows in opposite directions. The magnetic field inside and outside the coaxial cable is determined by using Ampère's law. The magnetic field inside...
2.1K
03:25Chemistry of Carbohydrates
90.9K
Carbohydrates are an essential part of the diet in humans and animals. Grains, fruits, and vegetables are natural sources of carbohydrates that provide energy to the body, particularly through glucose, a simple sugar that is a component of starch and an ingredient in many staple foods. The stoichiometric formula (CH2O)n, where n is the number of carbons in the molecule represents carbohydrates. In other words, the ratio of carbon to hydrogen to oxygen is 1:2:1 in carbohydrate molecules. This...
90.9K