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Within-a-Day Detection and Rapid Characterization of Carbapenemase by Use of a New Carbapenem Inactivation

François Caméléna1,2, Aurélie Cointe1,3, Vincent Mathy3

  • 1IAME, UMR 1137, INSERM, Université Paris Diderot, Sorbonne Paris Cité, Paris, France.

Journal of Clinical Microbiology
|June 29, 2018
PubMed

Insights

The new CIMplus test rapidly detects carbapenemase-producing Enterobacteriaceae (CPE) in 8 hours and identifies carbapenemase classes in 20 hours. This simple, accurate tool aids in controlling the spread of CPE infections.

Area of Science:

  • Clinical Microbiology
  • Infectious Diseases
  • Antimicrobial Resistance

Background:

  • Carbapenemase-producing Enterobacteriaceae (CPE) pose a significant public health threat.
  • Rapid detection of CPE is crucial for effective treatment and infection control.
  • The existing carbapenem inactivation method (CIM) has a 20-hour detection time.

Purpose of the Study:

  • To evaluate the performance of the CIMplus test for rapid detection and characterization of carbapenemases.
  • To assess the CIMplus test's accuracy and speed compared to existing methods.
  • To determine the utility of CIMplus for identifying carbapenemase classes.

Main Methods:

  • The CIMplus test was evaluated using 110 carbapenem-resistant Enterobacteriaceae strains, including 92 CPE isolates.
  • Carbapenemase activity was detected at 8 and 20 hours.
  • Carbapenemase classes were identified using specific inhibitors and a decision algorithm.

Main Results:

  • CIMplus demonstrated high sensitivity (95.7% at 8h, 97.8% at 20h) and specificity (94.4%).
  • Carbapenemase activity was detected within 8 hours.
  • Carbapenemase class identification was successful for 98.9% of CPE isolates, with high accuracy for Ambler classes A, B, and D.

Conclusions:

  • The CIMplus test provides rapid detection of carbapenemase activity in 8 hours.
  • It accurately characterizes carbapenemase classes within 20 hours.
  • CIMplus is a simple, affordable, and equipment-free tool for CPE detection and characterization.
Keywords:
EDTAEnterobacteriaceaeNDMOXA-48carbapenemasecarbapenemase inactivation methodmCIMphenotypic characterizationphenotypic detectionphenylboronic acid

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