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Published on: January 19, 2019
DNA Block Macromolecules Based on Rolling Circle Amplification Act as Scaffolds to Build Large-Scale Origami
Zhiqing Zhang1, Hongzhi Zhang1, Fang Wang1
1College of Science, China University of Petroleum, No. 66, West Changjiang Road, Qingdao, 266580, China.
Abstract:
A simplified origami strategy to create large-scale complex DNA nanostructures based on the rolling circle amplification (RCA) is developed. The long repetitive block single strand DNA (ssDNA) synthesized from RCA with a few staple strands are used to fabricate DNA origami, which can be assembled into the first level assemblies of micro-scale length mono-nanoladders. Depending on base pairing among the sticky ends of short staple strands in nanoladders, the second level assemblies with large-scale and controlled structures such as tri-nanoladder, penta-nanoladder, and even nanobrocade, can be further achieved.
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