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Anti-thyroid peroxidase antibody in patients with autoimmune thyroid disease: possible identity with anti-microsomal
Abstract:
Thyroid microsomal antigen and peroxidase (TPO) have a close intracellular anatomical relationship, especially in exocytotic vesicles. We considered that antibodies to microsomal antigen might react with TPO and therefore looked for the presence of antibodies against TPO in the serum of patients with autoimmune thyroid disease (AITD). TPO was prepared from Graves' thyroid glands, solubilized by n-octyl glucoside, and its activity was assayed by the guaiacol method. Control sera and sera with a positive microsomal hemagglutination test (MCHA(+) ) were assayed for their ability to precipitate TPO activity by incubation of sera with TPO and protein A. We identified MCHA(+) sera which caused precipitation of TPO activity, and the extent of precipitation was related to the amount of serum added. A significant correlation was present between this anti-peroxidase activity and microsomal antibodies titers, measured by a micro-ELISA method. Affinity columns prepared from immunoglobulins of MCHA(+) sera, coupled to Reacti-Gel (6X), bound TPO activity, whereas using control IgG the recovery in the unbound fraction was high. These data provide evidence of antibodies against thyroid peroxidase in the serum of patients with AITD and suggest a close link between microsomal antigen and thyroid peroxidase.
Insights
Researchers found antibodies against thyroid peroxidase (TPO) in patients with autoimmune thyroid disease (AITD). This suggests a strong link between microsomal antigen and TPO, crucial for understanding thyroid autoimmunity.
Area of Science:
- Immunology
- Endocrinology
- Molecular Biology
Background:
- Thyroid microsomal antigen and thyroid peroxidase (TPO) share intracellular proximity.
- Autoimmune thyroid disease (AITD) is characterized by immune responses against thyroid components.
Purpose of the Study:
- To investigate the presence of antibodies against TPO in patients with AITD.
- To explore the relationship between microsomal antigen antibodies and TPO.
Main Methods:
- Preparation and solubilization of TPO from Graves' thyroid glands.
- Assay of TPO activity using the guaiacol method.
- Detection of anti-TPO activity in patient sera via precipitation and affinity chromatography.
Main Results:
- Sera positive for microsomal hemagglutination test (MCHA(+)) precipitated TPO activity.
- A significant correlation was observed between anti-peroxidase activity and microsomal antibody titers.
- Affinity columns using MCHA(+) immunoglobulins effectively bound TPO activity.
Conclusions:
- Evidence supports the presence of antibodies against TPO in AITD patients.
- A close link between microsomal antigen and TPO is suggested in the pathogenesis of AITD.