Related Experiment Video
Updated: Feb 8, 2026

Optimized Interferon-gamma ELISpot Assay to Measure T Cell Responses in the Guinea Pig Model after Vaccination
Published on: January 20, 2019
Towards a Full Automation of the ELISpot Assay for Safe and Parallelized Immunomonitoring
J C Neubauer1,2, I Sébastien2, A Germann1
1Fraunhofer Institute for Biomedical Engineering, Sulzbach, Germany.
Abstract:
The ELISpot assay, as a sensitive and specific method, enables the detection of cytokines for immunological purposes and in vaccine development. Here we describe the successful transfer of the manual procedure to a commercially available automated liquid handling platform, based on the work described in Neubauer et al. (Cytotechnology 69:57-73, 2017). Different kinds of technical issues (dead volume reduction, instrumental handling limitations, liquid class improvement) have been solved and biological effects (reagents concentration, selectivity tests, dispensing way, etc.) have been controlled during the implementation process. At the end a maximum of 6% mean delta difference and a lower mean dispersion than the manual assay were reached as well as a turnaround time of four to six times higher than the manual process.
Related Concept Videos
Parallel Resonance
Parallel Processing
Guidelines and Strategies for Safe Computer Charting
Maintain Confidentiality and Security:
Resistors In Parallel
Series and Parallel Capacitors
First, consider capacitors connected in series to a battery. In this configuration, the plate connected to the battery's positive terminal develops a positive charge, while the plate attached to the negative terminal becomes negatively charged. An equal magnitude of charge is induced on the...
Parallel-axis Theorem

