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Atomic Force Microscopy Imaging and Force Spectroscopy of Supported Lipid Bilayers
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Atomic Force Microscopy Imaging and Force Spectroscopy of Supported Lipid Bilayers

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Supported Lipid Bilayers for Atomic Force Microscopy Studies.

Zhengjian Lv1,2, Siddhartha Banerjee1, Karen Zagorski1

  • 1Department of Pharmaceutical Sciences, University of Nebraska Medical Center, Omaha, NE, USA.

Methods in Molecular Biology (Clifton, N.J.)
|June 30, 2018
PubMed
Summary
This summary is machine-generated.

We developed protocols for creating stable, smooth supported lipid bilayers (SLBs) using POPC and POPS lipids. These defect-free SLBs are ideal for hours-long atomic force microscopy (AFM) studies of biological molecules.

Keywords:
Amyloid aggregationAtomic force microscopeNanoimagingSupported lipid bilayerTime-lapse imaging

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Area of Science:

  • Biophysics
  • Materials Science

Background:

  • Supported lipid bilayers (SLBs) are crucial models for cellular membranes in biophysical studies.
  • Atomic force microscopy (AFM) requires stable, smooth SLBs for effective imaging of molecular interactions.
  • Existing SLB preparation methods often lack the stability and morphology needed for extended AFM observations.

Purpose of the Study:

  • To establish reliable protocols for assembling defect-free supported lipid bilayers (SLBs) using specific phospholipids.
  • To ensure the stability and smooth morphology of SLBs for prolonged nanoimaging applications.
  • To demonstrate the utility of these enhanced SLBs in studying dynamic biological processes like protein aggregation.

Main Methods:

  • Utilized 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine (POPS) for SLB formation.
  • Employed mica as a support substrate for SLB assembly.
  • Applied time-lapse atomic force microscopy (AFM) for surface morphology analysis and dynamic monitoring.

Main Results:

  • Successfully assembled defect-free POPC and POPS SLBs on mica supports.
  • Demonstrated that the prepared SLBs exhibit stability for a minimum of 8 hours.
  • Validated the application of these smooth and stable SLBs by successfully monitoring amyloid protein aggregation using time-lapse AFM.

Conclusions:

  • The developed protocols yield high-quality SLBs suitable for advanced nanoimaging techniques.
  • Stable and smooth SLBs are essential for long-term AFM studies of membrane-associated biological phenomena.
  • This methodology provides a robust platform for investigating molecular interactions and dynamics at the membrane interface.