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High-Speed Atomic Force Microscopy of Individual Amyloidogenic Protein Assemblies
Takahiro Watanabe-Nakayama1, Kenjiro Ono2
1WPI Nano Life Science Institute (WPI-NanoLSI), Kanazawa University Kakuma-machi, Kanazawa, Japan. tnakawata@se.kanazawa-u.ac.jp.
Abstract:
High-speed atomic force microscopy (HS-AFM) with high spatiotemporal resolution allows for the video imaging of the conformational changes of individual molecules in an observation area in liquid at nanometer-scale spatial resolution. This method verifies the molecular mechanism and reveals the structural dynamics of relevant biomolecules for various biological phenomena. Here, we describe the methods for HS-AFM observation and the analysis of the structural dynamics of individual amyloidogenic protein assemblies using amyloid β 1-42 as an example.
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