Related Experiment Video
Updated: Feb 8, 2026

Quantitative Hardness Measurement by Instrumented AFM-indentation
Published on: November 22, 2016
AFM Indentation Analysis of Cells to Study Cell Mechanics and Pericellular Coat
Igor Sokolov1,2,3, Maxim E Dokukin4
1Department of Mechanical Engineering, Tufts University, Medford, MA, USA. Igor.sokolov@tufts.edu.
Abstract:
Atomic force microscopy (AFM) indentation analysis of cells is a unique method of measuring stiffness of the cell body and physical properties of its pericellular coat. These cell parameters correlate with cells of abnormality and diseases. Viable biological cells can be studied with this method directly in a culture dish with no special preparation. Here we describe a step-by-step method to analyze the AFM force-indentation curves to derive cell mechanics (the modulus of elasticity of the cell body) and the parameters of the pericellular coat (density and the thickness of the coat layer). Technical details, potential difficulties, and points of special attention are described.
Related Concept Videos
Adaptive Mechanisms in Cancer Cells
Some of the advantages that cancer cells have on normal cells include - enhanced ability to divide without terminally differentiating, induce new blood vessel formation,...
Cell-matrix's Response to Mechanical Forces
Anchoring junctions mechanically attach a cell to the...
Pinching-off of Coated Vesicles
Clathrin Coated Vesicles
COP Coated Vesicles
Coat Assembly and GTPases
Coat assembly depends on the local availability of phosphatidylinositol phosphates or PIPs and GTP-binding proteins. Adaptor proteins, which link the coat proteins to the membrane, bind to these PIPs and play a crucial role in controlling...

