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Updated: Feb 8, 2026

Modified Yeast-Two-Hybrid System to Identify Proteins Interacting with the Growth Factor Progranulin
Published on: January 17, 2012
Analysis of Progranulin-Mediated Akt and MAPK Activation
Shi-Qiong Xu1, Simone Buraschi2, Ryuta Tanimoto1
1Biology of Prostate Cancer Program, Department of Urology, Thomas Jefferson University, Philadelphia, PA, USA.
Abstract:
Progranulin has emerged in recent years as an important regulator of various biological functions including cell proliferation, wound healing, motility, and protection from apoptosis. Progranulin is also critical for transformation as established in several cancer models.Progranulin biological responses elicit through the activation of the Akt and MAPK pathways, which are critical for progranulin downstream signaling.In this chapter various experimental approaches aiming at detecting progranulin-mediated Akt and MAPK activation will be discussed.
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