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Decreased expression of TROAP suppresses cellular proliferation, migration and invasion in gastric cancer

Ke Jing1, Qinsheng Mao1, Peng Ma1

  • 1Department of Gastrointestinal Surgery, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, P.R. China.

Molecular Medicine Reports
|June 30, 2018
PubMed

Insights

Trophinin associated protein (TROAP) is upregulated in gastric cancer, promoting cell proliferation and invasion. Knocking down TROAP suppressed these oncogenic behaviors, suggesting it as a potential therapeutic target for gastric cancer.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Trophinin associated protein (TROAP) is a cytoplasmic protein involved in spindle assembly and cell invasion.
  • The specific role of TROAP in cancer development and progression is not fully understood.

Purpose of the Study:

  • To investigate the expression and function of TROAP in gastric cancer (GC).
  • To determine the potential of TROAP as a prognostic marker and therapeutic target in GC.

Main Methods:

  • Analysis of TROAP mRNA expression in GC tissues using Oncomine database.
  • Kaplan-Meier survival analysis to correlate TROAP expression with patient outcomes.
  • In vitro experiments involving TROAP knockdown in GC cell lines (SGC-7901, MGC80-3).
  • Assessment of cell proliferation (MTT assay), cell cycle progression (flow cytometry), and migration/invasion (Transwell assay).

Main Results:

  • TROAP mRNA expression was significantly upregulated in gastric cancer tissues compared to normal tissues.
  • Elevated TROAP expression was associated with poorer survival rates in GC patients.
  • Knockdown of TROAP inhibited GC cell proliferation, suppressed G1 to S phase transition, and reduced migration and invasion capabilities.

Conclusions:

  • TROAP is overexpressed in gastric cancer and plays a significant oncogenic role.
  • TROAP promotes gastric cancer cell proliferation and invasion, indicating its potential as a therapeutic target.

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