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Semi-automated Imaging of Tissue-specific Fluorescence in Zebrafish Embryos
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Choosing the right microscope to image mitosis in zebrafish embryos: A practical guide
Iskra Yanakieva1, Marija Matejčić1, Caren Norden1
1Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.
Methods in Cell Biology
|June 30, 2018
Summary
Investigating cell division during development requires microscopy. This study presents protocols for observing mitosis in zebrafish embryos at multiple scales, balancing resolution and phototoxicity for comprehensive insights.
Area of Science:
- Developmental Biology
- Cell Biology
- Microscopy
Background:
- Orchestrated cell proliferation is essential for tissue growth and organismal development.
- Understanding cell division's role in development necessitates examining mitosis across tissue, cellular, and subcellular levels.
Purpose of the Study:
- To present three protocols for studying mitosis in developing zebrafish embryos.
- To guide the selection of appropriate microscopy techniques for different research questions regarding mitosis and development.
Main Methods:
- Utilizing zebrafish embryos for their transparency and rapid external development.
- Employing various microscopy approaches, including laser-scanning confocal, spinning disk confocal, and light-sheet fluorescence microscopy.
- Balancing temporal and spatial resolution with minimal phototoxicity for accurate observation.
Main Results:
- Demonstrated protocols for assessing mitotic index at the tissue level.
- Enabled cell lineage tracing at the cellular level.
- Facilitated characterization of intracellular components at the subcellular level.
Conclusions:
- Different microscopy techniques are crucial for addressing specific questions about mitosis in developing tissues.
- Choosing the right microscope ensures a comprehensive and unbiased view of how mitosis impacts development.
- This work provides a framework for robustly studying developmental cell division.
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