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Published on: May 4, 2014
Primary Cultures of Olfactory Neurons from the Avian Olfactory Epithelium
1Biology Department, University of Scranton, Scranton, PA, USA. george.gomez@scranton.edu.
Abstract:
The culture of the olfactory epithelium has been a useful model for the study of neurogenesis since olfactory neurons regenerate continuously throughout the adult lifespan. Structurally and functionally mature olfactory neurons are generated in vitro from non-neuronal precursors in a process that resembles the in vivo counterparts. This chapter describes a technique for culture of olfactory neurons from the avian olfactory epithelium of embryonic chickens; this enables the controlled laboratory study of a critical sensory system that is unstudied in this major vertebrate class. The techniques described here are broadly applicable to other endothermic vertebrate species.
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