Related Experiment Video
Updated: Feb 8, 2026

Development of Sulfidogenic Sludge from Marine Sediments and Trichloroethylene Reduction in an Upflow Anaerobic Sludge Blanket Reactor
Published on: October 15, 2015
[Enrichment of Nitrospira in Activated Sludge and Kinetic Characterization]
Qian Yao1, Dang-Cong Peng1, Qiao-di Zhao1
1School of Municipal and Environmental Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China.
Abstract:
Nitrospira was enriched from activated sludge by using fed-batch cultivation, and its related kinetic characterization was studied. The results showed that Nitrospira could be successfully enriched from the activated sludge for a controlled nitrite concentration no higher than 2 mg·L-1. The maximum nitrate oxidation rate was 48.72 mg·(g·h)-1. The fluorescence for the in situ hybridization results showed that Nitrospira accounted for about 75% of the total biomass, while Nitrobacter accounted for only 0.1%. In addition, the kinetic parameters of Nitrospira at 20℃ were also investigated. The results showed that the optimum growth temperature for Nitrospira was 30-35℃. The temperature correction coefficient τN was 1.046. The nitrite half-saturation constant KS and oxygen half-saturation constant KO were (0.32±0.03)mg·L-1 and (1.52±0.09)mg·L-1, respectively. This study on the kinetic characterization of Nitrospira provided a theoretical foundation for the treatment plant design and process optimization.
More Related Videos
Related Concept Videos
Kinetic Energy
Enzyme Kinetics
Scientists typically study enzyme kinetics with a fixed amount of enzyme in the controlled environment of a test tube. When more reactant, or substrate, is...
Kinetic Molecular Theory: Molecular Velocities, Temperature, and Kinetic Energy
Kinetic Friction
Elimination Kinetics: First-Order and Zero-Order
Drug clearance depends on the rate of drug elimination and its plasma concentration. Another important parameter is a drug's half-life, which is the time required for its concentration to decrease by half. In most cases, drug clearance follows first-order...
Kinetic Energy - I

