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Updated: Feb 8, 2026

Quantification of Metal Leaching in Immobilized Metal Affinity Chromatography
Published on: January 17, 2020
[Comparison of Different Leaching Methods for Heavy Metals in Sludge Fly Ash and Comprehensive Toxicity Evaluation]
Feng Wang1, Run-Dong Li1, Yan-Long Li1
1Key Laboratory of Clean Energy, College of Energy and Environment, Shenyang Aerospace University, Shenyang 110136, China.
Abstract:
Fly ash from sludge incineration was separated into five different sizes (<1 μm, 1-2.5 μm, 2.5-10 μm, 10-50 μm, and > 50 μm) by high-precision air classification equipment. The leaching of heavy metals was contrastively studied using the HJT 299-2007-sulfuric acid/nitric acid method, HJ 557-2009-Horizontal Oscillation Method, toxicity characteristic leaching procedure (TCLP), and European standard protocol (EN 12457-3) for the different size fractions of the fly ash. Based on the leaching results, an evaluation method for the comprehensive toxicity of heavy metal leaching was established. The results show that the content of heavy metals and the amount of leaching from the fly ash decrease with the increase in fly ash particle size. The leaching of the heavy metals Zn and Cu in the < 1 μm particle size range of TCLP leaching method was the highest, at 107.34 mg·kg-1 and 318 mg·kg-1, respectively. The TCLP and sulfuric acid/nitric acid methods of heavy metal leaching were more effective than the EU (EN 12457-3) and horizontal oscillation methods. According to the value of OPTI, the OPTI value of < 10 μm fly ash was much larger than that of fly ash that was > 10 μm. This indicated that the fly ash of particle size < 10 μm was more toxic and more harmful.
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