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Immunohistochemistry in Investigative and Toxicologic Pathology
Kyathanahalli S Janardhan1, Heather Jensen2, Natasha P Clayton2
11 Integrated Laboratory Systems Inc., Morrisville, North Carolina, USA.
This review details immunohistochemistry (IHC) techniques, focusing on factors influencing results and reproducibility. It offers practical guidance on antibody selection and method optimization for reliable pathology findings.
Area of Science:
- Pathology
- Immunohistochemistry
- Laboratory Techniques
Background:
- Immunohistochemistry (IHC) is a crucial diagnostic tool in pathology.
- Reproducibility and accurate interpretation of IHC results are essential for clinical decisions.
Purpose of the Study:
- To provide a comprehensive overview of IHC technical aspects.
- To discuss variables impacting IHC results, interpretation, and reproducibility.
- To offer practical guidance for antibody selection and method optimization.
Main Methods:
- Review of technical aspects of immunohistochemistry.
- Analysis of variables affecting IHC outcomes.
- Compilation of antibody performance data in mouse and rat tissues.
- Presentation of an IHC method optimization strategy.
Main Results:
- Identification of key variables influencing IHC results and reproducibility.
- Provision of a curated list of validated and non-validated antibodies for rodent tissues.
- Development of a systematic approach for IHC method optimization.
Conclusions:
- Standardizing IHC protocols is critical for reliable diagnostic outcomes.
- Careful antibody selection and method optimization enhance IHC accuracy.
- Transparent reporting of IHC methods in publications is necessary for reproducibility.
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