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Related Experiment Video

Updated: Feb 8, 2026

Super-Resolution Imaging of Bacterial Secreted Proteins Using Genetic Code Expansion

Super-Resolution Imaging of Bacterial Secreted Proteins Using Genetic Code Expansion

Published on: February 10, 2023

Switchable genome editing via genetic code expansion.

Toru Suzuki¹, Maki Asami¹, Sanjay G Patel²

¹Laboratory of Mammalian Molecular Embryology, Department of Biology and Biochemistry, University of Bath, Bath, BA2 7AY, England.

Scientific Reports

|July 4, 2018

Summary

This summary is machine-generated.

Researchers developed a novel CRISPR-Cas9 system for heritable mammalian genome editing. This system is precisely controlled by Lys(Boc) (BOC), enabling precise gene editing applications.

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Area of Science:

Molecular Biology
Genetics
Biotechnology

Background:

CRISPR-Cas9 genome editing requires stringent regulation for safe application.
Existing controllable Cas9 variants face limitations in clinical and environmental settings.
Need for precise, heritable genome editing with external control mechanisms.

Purpose of the Study:

To develop a heritable CRISPR-Cas9 genome editing system controllable by a small molecule.
To enable precise spatiotemporal control over mammalian genome editing.
To create a foundation for environmentally compatible gene drives.

Main Methods:

Utilized genetic code expansion for non-physiological incorporation of Lys(Boc) (BOC).
Engineered a Cas9 variant (Cas9^BOC) active only upon BOC exposure.
Expressed Cas9^BOC during mouse embryonic development for heritable editing.

Main Results:

Cas9^BOC was expressed in a full-length, active form in somatic cells post-BOC exposure.
Demonstrated BOC-dependent, heritable editing of both transgenic and native genomic loci.
Achieved precise control over genome editing initiation during early mouse development.

Conclusions:

Developed a novel, heritable, and tightly controlled CRISPR-Cas9 genome editing system.
The Lys(Boc) (BOC) system offers a practical solution for therapeutic and gene drive applications.
This technology represents a significant step towards regulated spatiotemporal gene drives.