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Author Spotlight: A Streamlined and Accessible Analysis Method to Quantify Optokinetic Reflex Tracking Responses
Published on: April 12, 2024
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Development of optokinetic tracking software for objective evaluation of visual function in rodents
Francisco Segura1,2, Justo Arines3, Ana Sánchez-Cano1,2
1Department of Applied Physics, University of Zaragoza, Zaragoza, Spain.
Scientific Reports
|July 4, 2018
Summary
This study developed automated software for optokinetic tracking assessments, improving objectivity. The software achieved high agreement with human experimenters in evaluating rodent visual function.
Area of Science:
- Neuroscience
- Biomedical Engineering
- Ophthalmology
Background:
- Optokinetic tracking is crucial for assessing visual function in animal models.
- Current methods often rely on subjective human observation, limiting objectivity.
- Pigmented P23H rats serve as a model for retinitis pigmentosa, a degenerative eye disease.
Purpose of the Study:
- To develop and validate automated software for objective optokinetic tracking.
- To enhance the reliability and reproducibility of visual function assessments.
- To analyze visual function decline in a rodent model of retinitis pigmentosa.
Main Methods:
- Development of image processing software using MATLAB.
- Automated analysis of optokinetic responses in videos.
- Comparison of software results with assessments by two human experimenters.
- Testing on normal animals and pigmented P23H rats.
Main Results:
- The software achieved a 78% agreement rate with human experimenters, including direction of rotation.
- False positive rate was 7%, false negative rate was 10%, and overall error rate was 5%.
- Age-related decrease in visual function was observed in pigmented P23H rats.
Conclusions:
- The developed software provides an objective and reliable method for optokinetic tracking.
- Automated assessment significantly enhances the objectivity of visual function testing in rodents.
- The software is effective in detecting subtle head movements indicative of visual perception.
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